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 Table of Contents  
ORIGINAL ARTICLE
Year : 2016  |  Volume : 16  |  Issue : 2  |  Page : 159-166

An in vitro comparative study to evaluate the retention of different attachment systems used in implant-retained overdentures


Department of Prosthodontics and Crown and Bridge, VS Dental College and Hospital, Bengaluru, Karnataka, India

Date of Submission18-Jun-2015
Date of Acceptance11-Oct-2015
Date of Web Publication28-Mar-2016

Correspondence Address:
Tejomaya Shastry
Room No. 110, KIMS Men's Hostel, 18th Cross, 24th Main, Banashankari 2nd Stage, Bengaluru - 560 070, Karnataka
India
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0972-4052.176520

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  Abstract 

Aim: The aim of this in vitro study was to compare the change in the retentive force and removal torque of three attachment systems during simulation of insertion-removal cycles.
Methodology: Edentulous mandibular models were made with heat-cured polymethyl methacrylate resin. Two implant replicas (CMI), of 3.75 mm diameter and 10 mm length, were placed in the intraforaminal region. Acrylic resin mandibular overdentures were fabricated and provision was made to receive three different overdenture attachment systems, prefabricated ball/o-ring attachment (Lifecare Biosystems, Thane, India), Hader bar and clip attachment (Sterngold, Attleboro, MA), and Locator ® implant overdenture attachment stud type (Zest Anchors LLC, USA). Using a universal testing machine, each of the models were subjected to 100 pulls each to dislodge the overdenture from the acrylic model, and the force values as indicated on the digital indicator were tabulated both before and after thermocycling (AT).
Statistical Analysis Used: Statistical analysis comprised Kolmogorov-Smirnov test, Friedman test, and Wilcoxon signed ranks test.
Results: The statistical model revealed a significantly different behavior of the attachment systems both before and AT. The ball/o-ring and bar attachments developed higher retentive force as compared to the locator attachment. The bar and clip attachment exhibited the highest peak as well as the highest mean retention force at the end of the study. The Locator ® attachment showed a decrease in retentive potential after an early peak.
Conclusions and Clinical Implications: The ball/o-ring and bar and clip attachments exhibit higher retentive capacities than the Locator ® attachment over time.

Keywords: Dislodging cycles, Locator, overdenture attachment, retentive force, thermocycling


How to cite this article:
Shastry T, Anupama N M, Shetty S, Nalinakshamma M. An in vitro comparative study to evaluate the retention of different attachment systems used in implant-retained overdentures. J Indian Prosthodont Soc 2016;16:159-66

How to cite this URL:
Shastry T, Anupama N M, Shetty S, Nalinakshamma M. An in vitro comparative study to evaluate the retention of different attachment systems used in implant-retained overdentures. J Indian Prosthodont Soc [serial online] 2016 [cited 2019 Dec 6];16:159-66. Available from: http://www.j-ips.org/text.asp?2016/16/2/159/176520


  Introduction Top


The most common problem associated with the management of edentulous patients is the severely resorbed mandibular ridge, especially in older age when adaptive capacities are reduced. [1],[2],[3],[4],[5] This compromised situation consequently results in the fabrication of unsatisfactory dentures with poor retention and stability which can further precipitate psychosocial problems. [6],[7],[8],[9]

The stabilization of the lower denture with two interforaminal implants has provided reliable and predictable treatment outcomes. It is regarded as the minimum standard of care for edentulous patients. [10]

The prognosis of the prosthesis depends on two important factors: (1) Retention and (2) stress distribution. Retention is the function of and is directly related to the attachment system employed. The success of implant-retained overdentures primarily depends on the retentive capacity of its attachment element to sustain its long-term functionality. [11]

The choice of the attachment is dependent upon the retention required, jaw morphology, anatomy, mucosal ridge, oral function, and patient compliance for recall. [12]

Ball attachments and bar units for implant overdentures have evolved from the early 1960's. Ball attachments were considered the simplest type of attachments for clinical application with tooth- or implant-supported overdentures. [13] However, it is also well-documented that o-rings gradually loose retention, and must be replaced periodically. On the other hand, increased technique sensitivity and costs but with favorable stability have been reported regarding the bar attachments. Other disadvantages of the bar system include mucosal hyperplasia, hygiene problems, and the necessity of the retention clip's activation. [14],[15],[16]

The Locator ® attachment (Zest Anchors Inc., Homepage, Escondido, CA, USA) which was introduced in 2001, is a new system, which does not use the splinting of implants. This attachment is self-aligning and has dual retention in different colors with different retention values. [12],[17],[18] Locator ® attachments are available in different vertical heights, they are resilient, retentive, and durable, and have some built-in angulation compensation. In addition, repair and replacement are fast and easy. There is a lack of clinical studies on the Locator ® system. [19],[20]

Typically, the combination of materials in overdenture attachments comprises a metal-metal or metal-plastic/nylon contact which might show differences regarding surface wear and thus resistance to repetitive removal and insertion cycles. [21],[22]

In addition to this, a change in retentive capacity of the attachment systems is expected when the overdenture is subjected to a period of service in the oral cavity under the influence of inherently present fluids and ingested food and liquids during mastication and insertion and removal of the prosthesis. Micro- and macro-movement between the retentive surfaces of an attachment during mastication and removal of the overdenture will lead to wear and diminish retentive forces over time. [23]

Thus, the aim of this in vitro study was to test the hypothesis that the new unsplinted attachment system experiences less change of retentive force after repeated insertion-removal cycles compared to clinically established splinted attachment systems.

Aim of the study

The aim of this study was to assess and evaluate the retentive capacity of three most commonly employed attachment systems in implant-retained overdentures.

Objectives

  • To measure the retentive capacity of different implant overdenture attachment systems
  • To compare the retentive capacity of these attachment systems
  • To compare the change in the retentive force of different attachment systems during simulation of insertion-removal cycles.


Materials and equipment

  • Edentulous mandibular acrylic resin models made with heat polymerized polymethyl methacrylate resin - (DPI Heat Cure, DPI, Mumbai, Maharashtra, India)
  • Two implant replicas (CMI) - 3.75 mm diameter, 10 mm length [Figure 1]a.
    Figure 1: (a) Two implant replicas (CMI) - 3.75 mm diameter, 10 mm length. (b) Edentulous mandibular acrylic resin model with the two implant replicas placed in the intraforaminal region (22 mm apart) and retained with resin cement. (c) Mandibular overdenture fabricated in a conventional manner using heat polymerized polymethyl methacrylate resin

    Click here to view


Acrylic resin mandibular overdentures fabricated with heat polymerized polymethyl methacrylate resin - (DPI Heat Cure, DPI, Mumbai, Maharashtra, India).

  • Acrylic denture teeth - (Acryl-Rock)
  • Prefabricated ball/o-ring attachment (Lifecare Biosystems, Thane, India)
  • Hader bar and clip attachment (Sterngold, Attleboro, MA)
  • Locator ® implant overdenture attachment-stud type (Zest Anchors LLC, USA)
  • Resin cement (Relyx™, 3M ESPE)
  • Universal testing machine (UTM) - Instron 5567 compression tension tensile meter
  • Manual thermocycling unit - two S-U-Polytub, Schuler Dental, Germany
  • Surveyor table and metallic clips.



  Methodology Top


Fabrication of study models

Edentulous mandibular models were made from heat polymerized polymethyl methacrylate resin-(DPI Heat Cure, DPI, Mumbai, Maharashtra, India) [Figure 1]b.

Mandibular Overdentures were fabricated in a conventional manner using heat polymerized polymethyl methacrylate resin-(DPI Heat Cure, DPI, Mumbai, Maharashtra, India) [Figure 1]c.

Three overdenture models were prepared and five denture samples were prepared for each group.

  • Group 1 - Ball/o-ring attachment
  • Group 2 - Bar and clip attachment
  • Group 3 - Locator ® attachment.


The implant analogs (CMI 3.75 mm × 10 mm) were placed in the acrylic models using physiodispenser, simulating the conventional placement of implant in osteotomy site in the mandible and subsequently secured with resin cement (Relyx™, 3M ESPE, USA) [Figure 1]b.

IMPLANT OVERDENTURE ATTACHMENT SYSTEMS

  • Prefabricated ball/o-ring attachment (Lifecare Biosystems, Thane, India) [Figure 2]a
    Figure 2: (a) Prefabricated ball/o-ring attachment. (b) Bar attachment. (c) Locator attachment with various components

    Click here to view


    A metallic housing with a rubber o-ring component was used for the ball and ring attachment.
  • Hader bar and clip attachment [Figure 2]b

    A castable Hader bar of length = 22 mm; diameter = 1.8 mm = 13 gauge.

    Nylon rider-length = 5 mm; width = 2.6 mm - moderate retention
  • Locator ® attachment (Zest Anchors LLC, USA) [Figure 2]c Tissue cuff length = 1.0 mm; diameter = 3.86 mm

    Locator male blue inserts retention force = 1.5 lbs (6.7 N)

    Maximum convergence = 20°.


Each attachment system was secured into the implant replicas on the acrylic resin model and the overdentures with the corresponding housing were subsequently placed on it and tightened to 35 Ncm [Figure 3]a-f.
Figure 3: (a-c) Ball attachment, bar attachment, and Locator® attachment secured on to the implant replica on the acrylic resin model. (d-f) Acrylic resin overdenture with the o-ring housing for ball attachment, nylon ryder for the bar attachment, and the Locator male blue insert

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Experimental setup

Acrylic overdentures with respective attachment systems were placed on the acrylic edentulous mandibular models.

Metallic clips were attached to the dentures and secured with clear autopolymerized acrylic resin (DPI Cold Cure, Clear, DPI, Mumbai, Maharashtra, India).

The edentulous acrylic model was secured in place using a surveyor table [Figure 4].
Figure 4: Edentulous mandibular acrylic resin model and overdenture with clips attached secured in place using a surveyor table

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Retention force testing before thermocycling

With the UTM (Instron 5567 compression tension tensile meter), each of the models were subjected to 100 pulls each to dislodge the overdenture from the acrylic model, and the force values as indicated on the digital indicator were tabulated [Figure 5] and [Figure 6]. The dislodging force was applied in a vertical direction in the center of the acrylic block joining the two metallic clamps holding the overdenture with the UTM operating at a crosshead speed of 2 mm/30 ms. The readings were taken from the start of the test.
Figure 5: Universal testing machine - Instron 5567 compression tension tensile meter used to dislodge the overdentures from the models

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Figure 6: Digital values as seen on the universal testing machine

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Thermocycling

All the overdentures with the attachments placed on the edentulous models were subjected to manual thermocycling using S-U-Polytubs; one maintained at 5 ± 1° and other at 55 ± 1° [Figure 7]. The test samples were subjected to a total of 5000 cycles with each cycle equivalent to 30 s of dwell time in each temperature controlled tub with a transfer time of 10 s, with 5000 thermal cycles being equivalent to 6 months of service in the oral cavity. [24] None of the samples failed.
Figure 7: Manual thermocycling unit S-U-Polytub, Schuler Dental, Germany

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Retention force testing after thermocycling

Each of the models was again subjected to 100 pulls each to dislodge the overdenture from the acrylic model and the force values as indicated on the digital indicator were tabulated.


  Results Top


The Kolmogorov-Smirnov tests for normality revealed no normal distribution (P < 0.05) for the data; thus, normal distribution was not assumed.

Comparison of the repeated measures was performed using Friedman's test showing a statistically significant decrease in concentration.

In Group 1, χ2 (1) =30.556, P < 0.001. Post-hoc analysis with Wilcoxon signed-rank test was conducted with a Bonferroni correction applied, resulting in a significance level set at P < 0.001. The mean concentration (± standard deviation [SD]) was 56.26 (9.77) at baseline, 51.30 (5.08) at after thermocycling (AT). A significant decrease was seen between AT and baseline (Z = −5.969, P < 0.001) after the completion of 5000 thermal cycles [Table 1], [Table 2], [Table 3], [Table 4] and [Table 5].
Table 1: Friedman test descriptive statistics


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Table 2: Friedman test mean rank


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Table 3: Friedman test statistics


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Table 4: Wilcoxon signed ranks test-ranks


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Table 5: Wilcoxon test-statistics


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In Group 2, χ2 (1) =45.343, P < 0.001. Post-hoc analysis with Wilcoxon signed-rank test was conducted with a Bonferroni correction applied, resulting in a significance level set at P < 0.001. The mean concentration (±SD) was 70.66 (12.09) at baseline, 65.18 (10.89) at AT. A significant decrease was seen between AT and baseline (Z = −7.728, P < 0.001) [Table 1], [Table 2], [Table 3], [Table 4] and [Table 5].

In Group 3, χ2 (1) =17.640, P < 0.001. Post-hoc analysis with Wilcoxon signed-rank test was conducted with a Bonferroni correction applied, resulting in a significance level set at P < 0.001. The mean concentration (±SD) was 41.72 (6.53) at baseline, 36.74 (9.32) at AT. A significant decrease was seen between AT and baseline (Z = −4.446, P < 0.001) [Table 1], [Table 2], [Table 3], [Table 4] and [Table 5].

Interpretation

The bar and clip attachment showed the highest mean retentive force of 70.66 N and 65.18 N before and AT, respectively. The maximum retentive force was exhibited by the bar and clip attachment, 82.3 N (cycle no. 56); followed by Locator ® attachment, 66.7 N (cycle no. 41); and ball/o-ring attachment, 65.4 N (cycle no. 13). A decrease in the retention force was observed in all the three attachment systems after subjecting them to thermal cycles and this decrease was found to be statistically significant (P < 0.05).

The results obtained are summarized in [Table 6].
Table 6: Summary of statistical analysis


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  Discussion Top


The underlying principle in employing retentive implant-overdenture systems for the treatment of edentulous patients is to increase denture retention and stability, thereby promoting chewing function as well as patient comfort and compliance. [25],[26]

Stud type, ball, and conventional bar attachments are the commonly used anchorage systems in implant-supported overdentures and their efficacy is scientifically supported. [27],[28],[29],[30] Hence, these attachment systems were chosen for this study.

Splinted conventional bar attachments have demonstrated superior retentive capacities over unsplinted systems. However, they have a few disadvantages; they are initially more expensive, difficult to repair, and maintaining oral hygiene seems difficult, especially for fragile elderly individuals. [27]

In comparison with the bar attachments, ball anchors were preferred by clinicians because they were less technique sensitive, cost-effective, easy to use and to repair. [13]

Stud type attachments such as the Locator ® were introduced as a concept to simplify restorative procedures in implant-supported overdentures. This system is relatively easy in fabrication and demonstrated clinically superior results when compared with ball and bar attachments relative to prosthodontic complications and hygiene. [29]

This study was performed under a controlled experimental simulation to evaluate the retentive forces of three different types of anchorage systems used for implant-supported overdentures. The experimental set-up, however, may have had a few limitations. The sample size of the specimen used was relatively small, but was in accordance with previous similar experiments. [30]

It has to be kept in mind that for the current in vitro experiment, only mono-directional forces were applied, which does not represent a realistic model for a clinical situation with overdentures. There, the main forces are generated in the region of the first molars which will lead to rotational forces on the attachments through leverage. [31],[32],[33]

During the course of the study, the different attachments showed a complex evolution with peaks as well as increasing and/or decreasing mean retentive forces. The statistical model revealed a significantly different behavior of the attachment systems both before and AT [Figure 8] and [Figure 9].
Figure 8: Progression of mean retentive forces of the three attachment systems (each group n = 10) before thermocycling

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Figure 9: Progression of mean retentive forces of the three attachment systems (each group n = 10) after thermocycling

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The ball/o-ring and bar attachments developed higher retentive force as compared to the Locator ® attachments. The bar and clip attachment exhibited the highest peak as well as the highest mean retention force at the end of the study [Table 6].

The Locator ® attachment showed a decrease in retentive potential after an early peak.


  Conclusion Top


The ball/o-ring and bar-clip attachments maintain their retentive capacity longer than the Locator ® attachment.

A decrease in the retention force was observed in all the three attachment systems after subjecting them to thermal cycles and this decrease was found to be statistically significant.

Further research is required to understand the loss in retention force of various overdenture attachment systems.

Financial support and sponsorship

Nil.

Conflicts of interest

There are no conflicts of interest.

 
  References Top

1.
Mericske-Stern RD, Taylor TD, Belser U. Management of the edentulous patient. Clin Oral Implants Res 2000;11 Suppl 1:108-25.  Back to cited text no. 1
    
2.
Müller F, Heath R, Ott R. Maximum bite force after the replacement of complete dentures. Gerodontology 2001;18:58-62.  Back to cited text no. 2
    
3.
Müller F, Naharro M, Carlsson GE. What are the prevalence and incidence of tooth loss in the adult and elderly population in Europe? Clin Oral Implants Res 2007;18 Suppl 3:2-14.  Back to cited text no. 3
    
4.
Carlsson GE, Omar R. The future of complete dentures in oral rehabilitation. A critical review. J Oral Rehabil 2010;37:143-56.  Back to cited text no. 4
    
5.
Polzer I, Schimmel M, Müller F, Biffar R. Edentulism as part of the general health problems of elderly adults. Int Dent J 2010;60:143-55.  Back to cited text no. 5
    
6.
Allen PF, McMillan AS, Walshaw D. A patient-based assessment of implant-stabilized and conventional complete dentures. J Prosthet Dent 2001;85:141-7.  Back to cited text no. 6
    
7.
Awad MA, Lund JP, Shapiro SH, Locker D, Klemetti E, Chehade A, et al. Oral health status and treatment satisfaction with mandibular implant overdentures and conventional dentures: A randomized clinical trial in a senior population. Int J Prosthodont 2003;16:390-6.  Back to cited text no. 7
    
8.
Heckmann SM, Heussinger S, Linke JJ, Graef F, Pröschel P. Improvement and long-term stability of neuromuscular adaptation in implant-supported overdentures. Clin Oral Implants Res 2009;20:1200-5.  Back to cited text no. 8
    
9.
Müller F, Hernandez M, Grütter L, Aracil-Kessler L, Weingart D, Schimmel M. Masseter muscle thickness, chewing efficiency and bite force in edentulous patients with fixed and removable implant-supported prostheses: A cross-sectional multicenter study. Clin Oral Implants Res 2012;23:144-50.  Back to cited text no. 9
    
10.
Thomason JM, Feine J, Exley C, Moynihan P, Müller F, Naert I, et al. Mandibular two implant-supported overdentures as the first choice standard of care for edentulous patients - The York Consensus Statement. Br Dent J 2009;207:185-6.  Back to cited text no. 10
    
11.
Rutkunas V, Mizutani H, Takahashi H, Iwasaki N. Wear simulation effects on overdenture stud attachments. Dent Mater J 2011;30:845-53.  Back to cited text no. 11
    
12.
Evtimovska E, Masri R, Driscoll CF, Romberg E. The change in retentive values of locator attachments and hader clips over time. J Prosthodont 2009;18:479-83.  Back to cited text no. 12
    
13.
Alsabeeha NH, Payne AG, Swain MV. Attachment systems for mandibular two-implant overdentures: A review of in vitro investigations on retention and wear features. Int J Prosthodont 2009;22:429-40.  Back to cited text no. 13
    
14.
Sadowsky SJ. Mandibular implant-retained overdentures: A literature review. J Prosthet Dent 2001;86:468-73.  Back to cited text no. 14
    
15.
Payne AG, Solomons YF. Mandibular implant-supported overdentures: A prospective evaluation of the burden of prosthodontic maintenance with 3 different attachment systems. Int J Prosthodont 2000;13:246-53.  Back to cited text no. 15
    
16.
Gotfredsen K, Holm B. Implant-supported mandibular overdentures retained with ball or bar attachments: A randomized prospective 5-year study. Int J Prosthodont 2000;13:125-30.  Back to cited text no. 16
    
17.
Büttel AE, Bühler NM, Marinello CP. Locator or ball attachment: A guide for clinical decision making. Schweiz Monatsschr Zahnmed 2009;119:901-18.  Back to cited text no. 17
    
18.
Trakas T, Michalakis K, Kang K, Hirayama H. Attachment systems for implant retained overdentures: A literature review. Implant Dent 2006;15:24-34.  Back to cited text no. 18
    
19.
Chung KH, Chung CY, Cagna DR, Cronin RJ Jr. Retention characteristics of attachment systems for implant overdentures. J Prosthodont 2004;13:221-6.  Back to cited text no. 19
    
20.
Kleis WK, Kämmerer PW, Hartmann S, Al-Nawas B, Wagner W. A comparison of three different attachment systems for mandibular two-implant overdentures: One-year report. Clin Implant Dent Relat Res 2010;12:209-18.  Back to cited text no. 20
    
21.
Ludwig K, Cretsi X, Kern M. In vitro retention force changes of ball anchor attachments depending on divergences of implants. Dtsch Zahnarztl Ztg 2006;61:142-6.  Back to cited text no. 21
    
22.
Bayer S, Keilig L, Kraus D, Grüner M, Stark H, Mues S, et al. Influence of the lubricant and the alloy on the wear behaviour of attachments. Gerodontology 2011;28:221-6.  Back to cited text no. 22
    
23.
Rutkunas V, Mizutani H, Takahashi H. Evaluation of stable retentive properties of overdenture attachments. Stomatologija 2005;7:115-20.  Back to cited text no. 23
    
24.
Yao J, Li J, Wang Y, Huang H. Comparison of the flexural strength and marginal accuracy of traditional and CAD/CAM interim materials before and after thermal cycling. J Prosthet Dent 2014;112:649-57.  Back to cited text no. 24
    
25.
Doundoulakis JH, Eckert SE, Lindquist CC, Jeffcoat MK. The implant-supported overdenture as an alternative to the complete mandibular denture. J Am Dent Assoc 2003;134:1455-8.  Back to cited text no. 25
    
26.
Cune M, van Kampen P, van der Bilt A, Bosman F. Patient satisfaction and preference with magnet, bar-clip, and ball-socket retained mandibular implant overdentures: A cross-over clinical trial. Int J Prosthodont 2005;18:99-105.  Back to cited text no. 26
    
27.
Naert I, Gizani S, Vuylsteke M, Van Steenberghe D. A 5-year prospective randomized clinical trial on the influence of splinted and unsplinted oral implants retaining a mandibular overdenture: Prosthetic aspects and patient satisfaction. J Oral Rehabil 1999;26:195-202.  Back to cited text no. 27
    
28.
Karabuda C, Yaltirik M, Bayraktar M. A clinical comparison of prosthetic complications of implant-supported overdentures with different attachment systems. Implant Dent 2008;17:74-81.  Back to cited text no. 28
    
29.
Cakarer S, Can T, Yaltirik M, Keskin C. Complications associated with the ball, bar and Locator attachments for implant-supported overdentures. Med Oral Patol Oral Cir Bucal 2011;16:e953-9.  Back to cited text no. 29
    
30.
Kobayashi M, Srinivasan M, Ammann P, Perriard J, Ohkubo C, Müller F, et al. Effects of in vitro cyclic dislodging on retentive force and removal torque of three overdenture attachment systems. Clin Oral Implants Res 2014;25:426-34.  Back to cited text no. 30
    
31.
Steiner M, Ludwig K, Kern M. Retention forces of a new implant-supported bar attachment system. Clin Oral Implants Res 2009;20:1025-6.  Back to cited text no. 31
    
32.
Wiskott H. Bioengineering applied to oral implantology. Biomechanical studies. In: Ballo A, editor. Implant Dentistry Research Guide: Basic, Translational and Clinical Research. 1 st ed. Hauppauge, NY, USA: Nova Science Publishers; 2012. p. 369-426.  Back to cited text no. 32
    
33.
Yang TC, Maeda Y, Gonda T, Kotecha S. Attachment systems for implant overdenture: Influence of implant inclination on retentive and lateral forces. Clin Oral Implants Res 2011;22:1315-9.  Back to cited text no. 33
    


    Figures

  [Figure 1], [Figure 2], [Figure 3], [Figure 4], [Figure 5], [Figure 6], [Figure 7], [Figure 8], [Figure 9]
 
 
    Tables

  [Table 1], [Table 2], [Table 3], [Table 4], [Table 5], [Table 6]



 

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