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ORIGINAL STUDY COMPETITION
Year : 2018  |  Volume : 18  |  Issue : 5  |  Page : 13

OSC15: Tas1r1/Tas1r3 umami receptor expression levels are decreased in a serum starved muscle fiber model of atrophy


Date of Web Publication31-Oct-2018

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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0972-4052.244604

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How to cite this article:
. OSC15: Tas1r1/Tas1r3 umami receptor expression levels are decreased in a serum starved muscle fiber model of atrophy. J Indian Prosthodont Soc 2018;18, Suppl S1:13

How to cite this URL:
. OSC15: Tas1r1/Tas1r3 umami receptor expression levels are decreased in a serum starved muscle fiber model of atrophy. J Indian Prosthodont Soc [serial online] 2018 [cited 2018 Nov 20];18, Suppl S1:13. Available from: http://www.j-ips.org/text.asp?2018/18/5/13/244604



Miki Kobayakawa1,2, Tsuyoshi Nakajima2, William Addison3, Takuma Matsubara2, Shoichiro Kokabu2

1Division of Oral Reconstruction and Rehabilitation, Kyushu Dental University, Kitakyusyu, Japan, 2Division of Molecular Signaling and Biochemistry, Kyushu Dental University, Kitakyusyu, Japan, 3Research Unit, Shriners Hospitals for Children-Canada; Department of Human Genetics, McGill University, Montreal, Quebec, Canada

Aim: Properly controlled nutrition is an important factor for the successful outcome of dental implant treatments. Following digestion, excess amino acids absorbed from the intestine are stored in skeletal muscle. During starvation, this reservoir of amino acid can be released from skeletal muscle into the blood supply. In skeletal muscle tissue, Tas1r1/Tas1r3 has been shown to repress autophagy, and regulate amino acids metabolism. However, the exact mechanism by which Tas1r1/Tas1r3 regulates the storage and release of amino acids remains to be elucidated. Here, we report that the expression levels of Tas1r1 and Tas1r3 are decreased upon serum starvation of muscle fibers in vitro.

Materials and Methods: C2C12 cells were cultured and maintained in DMEM supplemented with 10% fetal bovine serum in 5% CO2. Single fibers of skeletal muscle and associated satellite cells were prepared from the extensor digitorum longus muscle of 8-week-old C57BL/6 mouse. To compare Tas1r1, Tas1r3, and Autophagy related genes expression in starved C2C12 cells, the datasets GSE925 were downloaded from the PubMed GEO and analysed.

Results: Tas1r1 and Tas1r3 was expressed by activated satellite cells, skeletal muscle stem cells and muscle fibers in vitro. The expression levels of Tas1r1/Tas1r3 in primary satellite cells increased with their differentiation. In silico and quantitative PCR analysis revealed that the expression levels of both Tas1r1 and Tas1r3 are decreased in serum-starved C2C12 cells.

Conclusion: Our data suggests that the reduction of Tas1r1/Tas1r3 induces autophagy and contributes to the release of amino acids into the blood stream from skeletal muscle tissue.






 

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