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ORIGINAL STUDY COMPETITION
Year : 2018  |  Volume : 18  |  Issue : 5  |  Page : 20

OSC29: Estimation of psychological and oxidative salivary stress markers in edentulous patients wearing complete dentures


Date of Web Publication31-Oct-2018

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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0972-4052.244620

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How to cite this article:
. OSC29: Estimation of psychological and oxidative salivary stress markers in edentulous patients wearing complete dentures. J Indian Prosthodont Soc 2018;18, Suppl S1:20

How to cite this URL:
. OSC29: Estimation of psychological and oxidative salivary stress markers in edentulous patients wearing complete dentures. J Indian Prosthodont Soc [serial online] 2018 [cited 2018 Nov 20];18, Suppl S1:20. Available from: http://www.j-ips.org/text.asp?2018/18/5/20/244620



Arunraj Daniel, Gopi Naveen Chander, Ramesh Reddy

SRM Dental College and Hospital, Bharathi Salai, Ramapuram, Chennai, Tamil Nadu- 600089

Purpose: The study was done to determine the levels of salivary alpha amylase (SAA), Thio barbituric acid reactive substances (TBARS), Nitric oxide (NO) in saliva during pre and post denture insertion.

Materials and Methods: Fifty patients were divided into two groups (A & B) for evaluating three biomarkers (SAA, TBARS, NO) in salivary samples. Un-stimulated saliva was collected at one time point from healthy dentate individuals (Group A) and at three different time intervals (before denture insertion, 1hour after denture insertion, one week after denture insertion) from edentulous individuals (Group B). Protocol was followed in sample handling, transport, and storage. The analysis of the SAA, TBARS and NO was done by biochemical enzymatic assay, lipid peroxidation and Griess reaction. The values obtained were statistically analysed using t-test.

Results: The results showed markers levels where different in the intervention group. The mean levels of SAA was 8.26 ± 1.32 min/ml during pre denture insertion and the decreased to 3.44± 1.25 min/ml in post denture insertion phase in the intervention group. TBARS 14.33± 4.71 nm/ml, NO 22.92 ± 5.79 ng/ml were increased in 1 week post denture insertion. The results were statistically significant with p < 0.001.

Conclusion: The psychological stress marker (SAA) decreased and Oxidative stress (TBARS, NO) increased with post denture insertion.






 

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