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 Table of Contents  
ORIGINAL STUDY COMPETITION
Year : 2018  |  Volume : 18  |  Issue : 5  |  Page : 25

OSC41: Preliminary study of the antibacterial effects of kaffir lime leaves on Streptococcus Mutans


Date of Web Publication31-Oct-2018

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DOI: 10.4103/0972-4052.244633

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How to cite this article:
... OSC41: Preliminary study of the antibacterial effects of kaffir lime leaves on Streptococcus Mutans. J Indian Prosthodont Soc 2018;18, Suppl S1:25

How to cite this URL:
... OSC41: Preliminary study of the antibacterial effects of kaffir lime leaves on Streptococcus Mutans. J Indian Prosthodont Soc [serial online] 2018 [cited 2020 Feb 28];18, Suppl S1:25. Available from: http://www.j-ips.org/text.asp?2018/18/5/25/244633



Farah Syazwani Mohamd Tarmizi1, Ho Ting Khee1, Aspalilah Alias2, Abd Rashid Li3, Natasya Ahmad Tarib1

1Restorative Centre, Faculty of Dentistry, University Kebangsaan Malaysia, 2Faculty of Dentistry, University Sains Islam Malaysia, 3Forest Research Institute of Malaysia

Aim: Kaffir lime has been reported to exhibit antibacterial and antioxidant effects. Streptococcus mutans has been considered the most important causative pathogen of dental caries. The aims of the present study were to investigate the potential antibacterial effects of water and tannin extracts of kaffir lime Citrus Hystrix leaves on S. mutans.

Materials and Methods: Water and tannin were extracted from kaffir lime. S. mutans was revived in brain-heart infusion (BHI) broth and incubated at 370C for 24 h. Two-fold serial dilution of test substances was prepared. Using a 96-well plate, 50 uL of each concentration of the diluted substances was added into 1 x 106 cfu/mL of S. mutans. After 24 h of incubation, the absorbance of the samples was measured at 560 nm using the microplate reader. The minimum inhibitory concentration (MIC) of the extract was determined, and cells from each well were cultured on BHI agar for minimum bactericidal concentration (MBC). All tested substances were compared with 0.12% chlorhexidine as positive control and BHI broth as negative control.

Results: Water and tannin extracted showed antibacterial activities against S. mutans with MIC ranged from 0.4 to 50.0 mg/ml. The MIC of water and tannin extracts were in the range of 1.5 – 12.0 mg/mL and 2.5 – 10.0 mg/mL, while their MBC were in the range of 3.0 – 6.0 mg/mL and 5.0 -10.0 mg/mL respectively.

Conclusions: The findings highlight the ability of kaffir lime leaves extract to inhibit S. mutans activity, which may be beneficial as a substance with the therapeutic features in decreasing dental carries.






 

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